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Fig. 8 Temozolomide treatment inhibits gliomagenesis in nf1 KO and nf1;tp53 cKO fish until 3 months of age. (A) Mortality analyses of nf1 KO and nf1;tp53 cKO fish with or without TMZ treatment (n = 100 for each group; two groups for each line). (B) Tumour incidences were significantly suppressed by TMZ treatment in nf1 KO and nf1;tp53 cKO fish at 3 months of age (n = 100 for each group; two groups for each line). (C) Representative immunohistochemistry images examining the expression of Pcna, pHH3, Gfap, Nestin, Cyclin D1, and pAkt in nf1 KO and nf1;tp53 cKO fish with or without TMZ treatment. Scale bars = 100 μm. (D) Quantification of the percentages of Pcna-positive cells in nf1 KO and nf1;tp53 cKO fish with or without TMZ treatment (n = 5 random fields of view). Data shown as mean ± SEM. *P < 0.05.