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Fig. 1 Sirt1 Knockout by Crispr/Cas9 technique.
(A) Schematic illustration of the Sirt1 locus. Exon 1 of the Sirt1 gene was targeted. The CRISPR target site and PAM motif are indicated. (B) Sequence confirmation of four-nucleotide insertion mutations. (C) Predicted truncation of the SIRT1 protein. (D) Expression of the Sirt1 mutant was verified by western blot using an antibody of Sirt1; β-actin was used as a loading control.
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