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FIGURE 3

Forebrain patterning is normal in bsx mutants. (a–p) Lateral views of 2 dpf zebrafish embryo heads stained by in situ hybridization using probes as indicated. No differences in expression of any marker was observed between wildtype and bsx mutant embryos. All images are minimum intensity projections of 40 brightfield focal planes (distance 1 μm). Anterior is to the left. Scale bar in (a) for all images: 50 μm. Anterior at left. Number n of embryos analyzed (wildtype) = 3 (a,e,h,i); 5 (b,m); 6 (c,p); 10 (d); 9 (f,n); 14 (g); 8 (j,k,o); 12 (l). n (bsx mutants) = 3 (a); 11 (b,g); 4 (c,j); 12 (d); 5 (e,l,o); 9 (f,n,p); 8 (h,i); 10 (k); 6 (m).

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