Fig. 2 pax1b is expressed in pharyngeal pouches but not in the neural crest cells of pharyngeal arches. Double fluorescence in situ hybridization indicated pax1b (A, red) is co-expressed with nkx2.3 (B, green) in pharyngeal pouches (pp) 2–6 (C, merge) at 36 hpf. pax1b (D, red) is also co-expressed with nkx2.7 (E, green) in pharyngeal pouches 3–6 (F, merge). Expression of pax1b (G, red) in the pharyngeal pouches is adjacent to dlx2a (H, green), which is expressed in the neural crest cells of pharyngeal arches (pa). Relative localization is apparent in the merged image (I). Scale bars represent 100 μm.
Reprinted from Mechanisms of Development, 161, Liu, Y.H., Lin, T.C., Hwang, S.L., Zebrafish Pax1a and Pax1b are required for pharyngeal pouch morphogenesis and ceratobranchial cartilage development, 103598, Copyright (2020) with permission from Elsevier. Full text @ Mech. Dev.