IMAGE

Figure 6

ID

ZDB-IMAGE-190723-1015

Genes

EGFP

Antibodies

Ab2-oxt

Source

Figures for Anbalagan et al., 2019

Image

Figure Caption

Figure 6 Specificity of antibodies to oxytocin and neurophysin.

(A) Schema of protein coded by oxt gene. Antibodies anti-OXT and PS45 were targeted to processed OXT and neurophysin respectively. SP denotes signal peptide, arrow indicates cleavage site. Validation of the EGFP-OXT fusion protein is shown in Figure 3H. (B–F) Confocal Z-stack images showing Fluorescent in situ hybdridization (FISH) of 3 days post-fertilization (dpf) larvae using probes for avp (B) or oxt (C) mRNA (red), followed by PS45 staining. The neurosecretory preoptic (NPO) area in which oxytocin neurons were labeled is shown. Confocal maximum intensity projection (MIP) images showing immunostaining of 5-dpf old transgenic Tg(oxt:EGFP) larvae using antibodies directed against GFP and neurophysin (NP). The whole neurohypophyseal tract (D) and the hypophyseal projections (E) are shown. Scale: 10 μM.

Figure Data

Expression / Labeling details

Acknowledgments

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