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Fig. 4
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Fig. 4
bif1 affects definitive hematopoiesis. (A) Bright-field images from O-dianisidine-stained embryos at 3 dpf. These embryos are injected with a control morpholino or a morpholino against bif1. (B) Percentage of embryos showing absent/low (gray) or high (black) levels of hemoglobin. Data are mean±s.e.m. (C-F) Whole-mount in situ hybridization for gata1 at 4 dpf (C), rag1 at 5 dpf (D), cmyb at 4 dpf (E), gata2b at 26 hpf, runx1 at 28 hpf and cmyb at 48 hpf (F), in embryos injected with control morpholino (ctrl-MO) or morpholino against bif1 (bif1-MO).
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