ZFIN Image: Dingare et al., 2018, Fig. 2

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Figure Caption

Fig. 2

Eggs from wwtr1^−/− females do not form a micropyle. (A-B′) The micropyle is stained by Coomassie Brilliant Blue in activated eggs from wwtr1^+/+ females (A,A′, n=10) but not from wwtr1^−/− females (B,B′, n=8). Arrows indicate the micropyle. (C-E) Blastula (arrowhead) or partially cleaved (black arrows) eggs from wwtr1^+/− (C) or wwtr1^−/− (D) females fertilized in vitro after enzymatic removal of the chorion and corresponding quantification (E). (F-I) WISH with the Balbiani body marker dazl (F-I, red arrow) and the animal pole marker ccnb1 (H,I, green arrow) in wwtr1^+/− (F,H, n=39) and wwtr1^−/− (G, I, n=31) oocytes at the indicated stage of oogenesis. FC nuclei are stained with DAPI. AP, animal pole; VP, vegetal pole. Scale bars: 50 μm in F-I; 500 μm in A-D.

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Acknowledgments

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