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Fig. 5
Zebrafish mfrp mutants show subretinal microglia/macrophages. (A, B, C) Cells in the subretinal space of mfrp mutant zebrafish stain positively with a microglia/macrophage marker (antibody 4C4; red arrows), whereas control heterozygous and wild-type sibling zebrafish lack such staining in the subretinal space. Resident microglia in the inner retina also stain with this marker (red arrowheads). (D, E) A pigmented subretinal cell, likely a macrophage, is visible among the photoreceptor outer segments in an mfrp mutant plastic section (red arrow). These pigmented cells are not seen in controls. Scale bars: (A–F) 100 μm; (G–H) 20 μm.
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Full text @ Invest. Ophthalmol. Vis. Sci.