ZFIN Image: Gallagher et al., 2017, Fig. S2

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Figure Caption

Fig. S2

her1 and dlc transcripts accumulate post-transcriptionally in pnrc2^oz22mutants. Exonic in situ probes reveal that segmentation clock-associated her1 and dlc transcripts are misexpressed in the expected one-quarter of embryos in a pnrc2^oz22 intercross, n=13/57 (X²=0.1, p=0.7) and n=8/30 (X²=0.04, p=0.83), respectively (A, B and D, E). Intronic in situ probes, however, reveal no differences in expression among embryos from the same clutch, n=30/30 (X²=10.0, p=0.0016) and n=45/45 (X²=15.0, p=0.0001), respectively (C, F). These results are consistent with previous observations in tor^b644 mutants using intronic and exonic in situ probes that distinguish nascent from processed transcripts (Dill et al, 2005).

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Reprinted from Developmental Biology, 429(1), Gallagher, T.L., Tietz, K.T., Morrow, Z.T., McCammon, J.M., Goldrich, M.L., Derr, N.L., Amacher, S.L., Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation, 225-239, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.