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Fig. 4
Analysis of NCC development in polr1c and polr1d mutant embryos.
(A-H) sox10 expression at 12 hpf and (I-P) foxd3 expression at 14 hpf reveal relatively normal patterns of early cranial NCC specification and migration in polr1c-/- and polr1d-/- embryos (black arrows). (Q-X) In contrast, dlx2 expression at 36 hpf reveals slightly diminished domains of activity in mutant embryos, particularly with respect to the posterior pharyngeal arches, which is suggestive of fewer mature NCC colonizing the pharyngeal arches. White arrows indicate pharyngeal arches 1 and 2. Scale bar = 200 μm.
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