Fig. 6

Fig. 6

mib gene is deleted in mibⁿⁿ²⁰⁰² mutants. (A) Diagram of the mutations of different mib alleles. The translation of mib in mibⁿⁿ²⁰⁰⁰ and mibⁿⁿ²⁰⁰¹ mutants stops at amino acids 5′ to ring finger domain 3 (RF3). (B) The linkage analysis result of mibⁿⁿ²⁰⁰² mutants. The upper part illustrates the locations of mib gene and the SSLP markers. The lower part demonstrates a close linkage of Z4662 and Z13620 to mib, with a recombination frequency of 0/97 and 2/214, respectively. (C) Real-time PCR results of mib expression in mibⁿⁿ²⁰⁰² homozygotes and WT embryos. The blue bars represent the expression level of mib in mibⁿⁿ²⁰⁰² homozygotes. The purple bars represent the expression level of mib in WT embryos. There is nearly no mib transcript detected in the mibⁿⁿ²⁰⁰² homozygotes. (D) WT embryos and (E) mibⁿⁿ²⁰⁰² mutants were stained with mib at 14 hpf, respectively. There is no mib mRNA detected in (E) mibⁿⁿ²⁰⁰² homozygous. (F) The results of mib genomic PCR were shown with fih (hif1an) as a positive control. AB represents AB WT samples; MU represents mibⁿⁿ²⁰⁰² homozygous samples; NE represents negative controls, where distilled water was used instead of genomic extracts as templates. The results showed that no mib genomic fragments are amplified.