ZFIN Image: Takeuchi et al., 2015, Fig. 4

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Figure Caption

Fig. 4

Rescue of the hematopoietic defects in lsd1^it627 embryos by etv2 knockdown. (A) Expression of gata1 in WT or lsd1^it627 embryos injected or not injected with 0.5 pmol of the indicated morpholino oligonucleotides. The down-regulation of gata1 in lsd1^it627 embryos (open arrowheads) was rescued by the etv2MO injection (closed arrowheads). 5mis, 5-mismatch. (B) Expression of pu.1 at 24 hpf in WT or lsd1^it627 embryos injected or not injected with 0.5 pmol of the indicated morpholino oligonucleotides. The gata1MO-induced ectopic pu.1 expression in the ICM (arrows) was lost in lsd1^it627 embryos (open arrowhead) and recovered by the coinjection of etv2MO (closed arrowhead). (C) Methylation status of histone H3-K4 on the etv2 gene in 15-hpf embryos injected (gray bars) or not injected (white bars) with 1 pmol of lsd1MO (lsd1MO inj). Genomic regions (up1, int2, 35bp, ex8) examined by ChIP are shown as black bars. The results are means ± SEs of three independent experiments. *P < 0.05; Student’s t test.>

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA