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Fig. 4 fscn1a is required for collective migration of the first NC stream.
Dorsal cranial views of wild type and fscn1a MZ embryos analyzed by RNA in situ hybridization for (A) foxd3 mRNA at 11 hpf, (B) sox10 mRNA at 15 hpf, showing asymmetric dispersion of NC cells in fscn1a MZ (arrow), and (C) dlx2a mRNA at 18, 28 and 36 hpf. Numbers correspond to pharyngeal arches, arrows indicate abnormal NC migration phenotypes observed in dlx2a+ NC cells in fscn1a MZ embryos. Anterior is left in all panels. Phenotypes shown in fscn1a MZ panels were observed in 100% (A) or ~20% (B-C) of embryos examined (n>200).
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