Fig. 1

Fig. 1

Knockdown of zebrafish eaf1 by the splicing–blocking morpholino (Eaf1–MO3) blocks hematopoiesis, but does not affect expression of early hematopoietic marker genes, except for spi1. (A) Knockdown of eaf1 reduced O-dianisidine staining of hemoglobin obviously at 36 hpf (b versus a). (B) The reduction of hemoglobin (b, stained by O-dianisidine) resulted from knockdown of eaf1 was partially rescued by co-injecting mismatch mRNA of eaf1 at 4.5 dpf (c). (C) Depletion of eaf1 did not alter the expression of scl (a and b), lmo2 (c and d) and gata2 (e and f) at 10 s in the posterior lateral mesoderm. (D) At 10s and 22 hpf, expression of gata1 was not affected by depletion of eaf1 in the posterior lateral mesoderm (a and b) and ICM (c and d). (E) At the 10s, depletion of eaf1 enhanced expression of spi1 in the anterior lateral mesoderm (a and b). (F) At 22 hpf, expression of spi1 was reduced by depletion of eaf1 (a–d). (G) At 22 hpf, the number of GFP-positive cells in spil::GFP transgenic zebrafish was reduced by depletion of eaf1 (a–d). hpf, hours post fertilization; dpf, days post fertilization; s, somite; ICM, intermediate cell mass. Eaf1–MO3, 4 ng/embryo; eaf1-mismatch mRNA, 600 pg/embryo.