ZFIN Image: Wong et al., 2012, Fig. 2

Image

Figure Caption

Fig. 2

Endocardial and myocardial markers are reduced or absent in cyclopamine (CyA) treated embryos compared to dimethylsulfoxide (DMSO)-treated controls. (A–D) Kdrl (A,B) and cdh5 (C,D)-expression within endocardial progenitors that migrate to the midline (arrow) is greatly reduced in CyA-treated embryos (B,D) compared to controls (A,C) at the 18-somite stage. Dorsal view of the anterior region, anterior is up. (E–J) Endocardial kdrl (E,F) and NFATc1 (G-J) expression (arrows) is greatly reduced or absent in CyA-treated embryos (F,H,J) compared to controls (E,G,I) at the 24 h (E–H) and 48 h (I,J) stages. (K,L) Myocardial cmlc2 expression (arrows) is reduced in CyA-treated embryos at the 18-somite stage. (E–L) Ventral view of deyolked flat-mounted embryos, anterior is up.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 361(2), Wong, K.S., Rehn, K., Palencia-Desai, S., Kohli, V., Hunter, W., Uhl, J.D., Rost, M.S., and Sumanas, S., Hedgehog signaling is required for differentiation of endocardial progenitors in zebrafish, 377-91, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.