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Fig. S4
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Fig. S4
Primordium patterning is disrupted following induction of Tg(hsp70l:dkk1-GFP). RNA in situ hybridization at 32 hpf to visualize expression of patterning markers in wild-type embryos (left panels) and Tg(hsp70l:dkk1-GFP) embryos heat-shocked at 28 hpf reveals that these factors are downregulated following Dkk1 transgene induction; lef1 (A,B), fgf10a (C,D) and pea3 (E,F). Expression of the chemokines cxcr4b (G,H) and cxcr7b (I,J) are indistinguishable between wild-type and heat-shocked Tg(hsp70l:dkk1-GFP) embryos. Scale bar: 20 μm.
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