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Fig. 4
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Fig. 4 The hypothalamic releasing hormone and dopamine signals are unaffected in usp39 mutants.
A–C and E–G: Whole-mount in situ hybridization with hypothalamic probes at 48 hpf, ventral view. (A–C) wild-type (wt) embryos. (E–G) usp39 mutant embryos. Expression of hypothalamic markers crh, gnrh2, and gnrh3 did not change. D and H: Immunocytochemistry with tyrosine hydroxylase (TH) antibody at 48 hpf, ventral view. (H) Expression of TH in usp39 mutant embryos did not change.
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