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Fig. 5 Motor neuron loss was observed in G93R os10 spinal cord at the disease end-stage. (A) ChAT immunohistochemistry of anterior spinal cord sections from adult G93R os10 fish and nTg siblings demonstrates reduced numbers of positively labeled motor neurons (denoted by arrows) in end-stage fish compared with controls. (B) ChAT-positive motoneurons (≥10 μm diameter) from anterior spinal cord sections (20 μm thickness) of adult end-stage fish were blinded to genotype and counted (*P<0.0001, n=40 sections per fish, three fish per genotype). Data are mean ± s.e.m. Bar, 200 μm.
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Full text @ Dis. Model. Mech.