ZFIN Image: Rai et al., 2010, Fig. 4

Image

Figure Caption

Fig. 4

Demethylase Activity Is Required to Maintain Progenitor Cell Populations in apc Mutants

(A) Whole-mount in situ staining for aldh1a2, fabp2, and hoxa13a in apc^mcr and apc^wt embryos injected with control Mo, aaa Mo, combined mbd4 and tdg Mo, or overexpression of dnmt1 as in Figure 1.

(B) Cross-sections of the intestines of apc^mcr and apc^wt embryos whole-mount in situ stained with aid (52 hpf) or mbd4 (72 hpf). Asterisks show pronephric ducts, and arrows point to the intestine. N, notochord; SC, spinal cord.

(C) MeDIP-qPCR for genes shown in the GFP-positive cells from gutGFP;apc^mcr and gutGFP;apc^wt embryos (72 hpf). FACS parameters and efficiency are shown in Figures S4A and S4B.

(D–F) Immunostaining (D), RT-PCR (E), and Me-DIP PCR (F) for the indicated genes in the intestines from adult wild-type zebrafish treated with DMSO or DEAB for 7 days. In (E), RT-PCR was performed on epithelial cells isolated from the intestinal crypts.

Error bars indicate ± SD. See also Figure S4.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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Reprinted from Cell, 142(6), Rai, K., Sarkar, S., Broadbent, T.J., Voas, M., Grossmann, K.F., Nadauld, L.D., Dehghanizadeh, S., Hagos, F.T., Li, Y., Toth, R.K., Chidester, S., Bahr, T.M., Johnson, W.E., Sklow, B., Burt, R., Cairns, B.R., and Jones, D.A., DNA demethylase activity maintains intestinal cells in an undifferentiated state following loss of APC, 930-942, Copyright (2010) with permission from Elsevier. Full text @ Cell