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Fig. 2 Analysis of nlgn expression levels during development. Expression levels were assayed by RT-PCR using primers specific to (A-G) the zebrafish nlgns across multiple developmental stages and in the adult brain, and (H) tubulin alpha control. From left to right, cDNA samples were derived from wild-type (AB/Tübingen) zebrafish at the 16-cell (1.5 hpf), 90% epiboly (9 hpf), 3-somite (11 hpf), 16-, 24-, 48-, 72-, and 7-dpf stages and from adult brain.
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