ZFIN Image: Kitaguchi et al., 2009, Fig. 4

Image

Figure Caption

Fig. 4 c/ebp1, pu.1 and runx1 coordinately regulate lyz expression. All embryos are around 24 hpf and lateral view; anterior is left. (A) Injection of mRNA mixture (runx1 [100 pg] + pu.1 [10 pg] or runx1 [100 pg] + c/ebp1 [100 pg]) did not affect lyz expression compared to uninjected embryos. lyz expression in the ICM (orange arrowhead) was weakly induced in the embryos injected with mRNA mixture (c/ebp1 [100 pg] + pu.1 [10 pg]). Injection of mRNA mixture (runx1 [100 pg] + c/ebp1 [100 pg] + pu.1 [10 pg]) strongly induced lyz expansion in the ICM (red arrowhead). (B) The expression of erythroid markers gata1 and band3 was comparable in both uninjected- and triple mRNA-injected embryos.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Mechanisms of Development, 126(5-6), Kitaguchi, T., Kawakami, K., and Kawahara, A., Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis, 314-323, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.