Fig. 1
Fig. 1 Expression of Nodal pathway genes in the lateral plate mesoderm. Confocal images of double fluorescent in situ RNA hybridization of spaw, lft2, and oep with respect to the midline marker shh and the heart marker cmlc2 at the 21-somite stage. Single channel expression (A-G and A″-G″) and overlay (A′-G′). All images are dorsal views of the embryo, anterior to the top and left to the left. Note broad expression of oep in left and right LPM (F and G) and more restricted expression of spaw in left non-cardiac LPM (F′ and F″) and lft2 in left cardiac LPM (B′ and B″). spaw is expressed adjacent to lft2 (E′).