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Figure Caption
Fig. 4 mRNA expression of fat during development and in adult tissues. Zebrafish β-actin was used as a loading control in separate reactions, on the same cDNA, and produces a 400 bp transcript. (A) Northern analysis detects a single 14 kb transcript from 12 hpf to adult. (B) RT-PCR was used to detect a 400 bp product indicating the presence of fat mRNA from 6 hpf through to adult. (C) RT-PCR detects a 400 bp fat mRNA product in most adult tissues.
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Reprinted from Gene expression patterns : GEP, 5(4), Down, M., Power, M., Smith, S.I., Ralston, K., Spanevello, M., Burns, G.F., and Boyd, A.W., Cloning and expression of the large zebrafish protocadherin gene, Fat, 483-490, Copyright (2005) with permission from Elsevier. Full text @ Gene Expr. Patterns