FIGURE

Fig. 4

ID

ZDB-FIG-240715-78

Publication

Miltenburg et al., 2024 - Characterisation of an Adult Zebrafish Model for SDHB-Associated Phaeochromocytomas and Paragangliomas

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Metabolomics, mitochondrial complex activity, and metanephrine levels of adult heterozygous sdhb mutant and WT zebrafish. All values are standardised relative to the associated WT mean and expressed in arbitrary units (a.u.). (A) Abundance of metabolites in muscle tissue of heterozygous sdhb mutant (n = 44) and WT (n = 49) zebrafish (n is the total of three replicates). Succinate levels and succinate/fumarate ratios were significantly increased in heterozygous sdhb mutants compared with their WT siblings. (B) Mitochondrial complex activity in heterozygous sdhb mutant (n = 3) and WT (n = 6) zebrafish. Activity levels for all mitochondrial complexes were not significantly different. CI-D: complex I; CI-Q: quinone; CII: complex II; CIII: complex III; COX: complex V/cytochrome c oxidase; SCC: cytochrome P-450; CS: citrate synthase. (C) Catecholamine metabolite levels in the swimming water of heterozygous sdhb mutant (n = 23) and WT (n = 12) zebrafish. 3-MT: 3-Methoxytyramine; NMN: Normetanephrine. Student’s t test for metabolomics analysis and catecholamine metabolite analysis, Mann–Whitney U test for mitochondrial complex activity analysis. * p < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sdhb^rmc200/+ (AB)
Observed In:	mitochondrial electron transport, succinate to ubiquinone muscle 3-methoxytyramine muscle asparagine muscle aspartate muscle fumarate(2-) muscle lactate muscle malate muscle Normetanephrine muscle succinate
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.