Fig. 5

Window-specific AA exposure resulted in differential behavioral responses. (A) A schematic diagram showing zebrafish embryos exposed to 0, 1, 4 and 16 μM AA from 8–24 hpf, or 1 and 4 μM from 48–72 hpf, and sampled at various time points for different motor behavior tests. (B) Spontaneous movement (tail bends/min) of embryos from 19 hpf to 25 hpf (n = 40). (C) Touch response of dechorionated embryos at 36 hpf (n = 24). (D, E) Locomotor response at 72 hpf (D) and 120 hpf (E). Black and white bars at the x-axis represent dark and light conditions, respectively. Dots are the mean activity (72 hpf) or speed (120 hpf) in 60 s intervals, and bars are mean activity or speed (mm/s) in 5-min intervals for each light condition (light or dark) (n = 24). Values are plotted as mean + SD. *P < 0.05 and **P < 0.001 indicate significant differences from the vehicle control.