FIGURE

Fig. 3

ID

ZDB-FIG-240621-7

Publication

Izquierdo Lafuente et al., 2024 - Vitamin B₁₂ uptake across the mycobacterial outer membrane is influenced by membrane permeability in Mycobacterium marinum

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Fig. 3

B12 is available to M. marinum during infection of zebrafish. Systemic infection of zebrafish embryo with M. marinum was established by injection of 50–200 CFU bacteria into the caudal vein. All bacterial strains carry a plasmid expressing RFP (tdtomato). The embryos were imaged at 4 days post-infection. (A) The level of infection was quantified by counting the pixels of red fluorescent signal per embryo. Strains: by WT, cobC::fs, cobC::fs-metH::fs, and cobC::fs-metE::fs; NI, noninfected. Significant changes in each group were determined by ANOVA testing. P > 0.05, not significant, not indicated. *P < 0.05; **P < 0.01; ***P < 0.001. (B) Representative pictures of the infected embryos; red pixels correspond to bacteria.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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