FIGURE
Figure 1—figure supplement 1.
- ID
- ZDB-FIG-240610-20
- Publication
- Zhang et al., 2024 - Enhancing CRISPR prime editing by reducing misfolded pegRNA interactions
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Figure 1—figure supplement 1.
Indel frequencies of non-refolded and refolded pegRNAs with SpCas9 in zebrafish. Indel frequencies in zebrafish induced by SpCas9 complexed with non-refolded or refolded pegRNAs at a molar ratio of 1:2 (1.8 µM of pegRNA). Target loci, PBS lengths, and pegRNA-specified edits are indicated at the bottom. Dots represent individual data points (n=3 biologically independent replicates, 5–10 embryos per replicate), bars the mean and error bars ± s.e.m. Results of unpaired two-tailed |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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