Fig. 3

Reduced oviposition and premature hatching in Gv knockout. Wildtype, wt; gnav1 deletion mutant, gnav1^−/−. Data for panels a) to e) are shown as whisker plots with quartile segments, mean values are indicated by crosses, individual measurements are shown as circles. (a) No difference in weight between 15 months adult wildtype and their homozygous gnav1 deletion mutant siblings. (b) No difference in body length between 15 months adult wildtype and their homozygous gnav1 deletion mutant siblings. (c) Clutch size (number of fertilised oocytes) is reduced in the homozygous gnav1 deletion mutant compared to their wildtype siblings exposed to the same mating schedule (9-month-old experienced breeding pairs, n = 10–13, p < 0.05). Unfertilized oocytes were rare. (d) No difference in survival rate at 24 hpf between wildtype and the homozygous gnav1 deletion mutant (sibling parents). After 1 dpf nearly no further death was observed for wildtype and mutant. (e) Hatching was evaluated early in the third day (54–56 hpf) and is faster in the homozygous gnav1 deletion mutant compared to wildtype (sibling parents) (p < 0.0001). Each circle represents a separate clutch. (f) Representative photographs of wildtype and gnav1 deletion mutant at 55 hpf, top pictures show a magnification for an unhatched embryo inside the chorion and a hatched fish. Red asterisks point out hatched larvae. Significance was estimated by two tailed unpaired t-test