FIGURE

Fig. 3

ID

ZDB-FIG-240415-3

Publication

Kasica et al., 2024 - Xanthohumol, a prenylated flavonoid from hops (Humulus lupulus L.) exerts multidirectional pro-healing properties towards damaged zebrafish hair cells by regulating the innate immune response

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Fig. 3

A set of microphotographs and a graph documenting inhibition of neutrophil migration towards 10 μM CuSO4 exposed hair cells resulting from co-treatment of 3 dpf Tg(MPX:GFP) larvae with XN. (a) The control larva presented normal distribution of neutrophils which were found in the ventral myotomes of the trunk and tail. (b) 10 μM CuSO4 exposure evoked the migration of the neutrophils towards the injured neuromasts (arrows). (c,d, e) XN co-treatment resulted in the dose-dependent inhibition of the neutrophil migration, which was reflected by a decreased number, or complete lack of the green fluorescent cells in the area of natural neuromast localization (arrows). The visualization was accomplished using a Zeiss LSM-700 confocal microscope (10× objective with 0.5 zoom). (f) the graph presenting the influence of XN on the total area covered by the neutrophils associated with L3, L4 and L5 neuromasts. XN decreased the number of the immune cells 1.5 fold (0.1 μM XN co-treated group), 3.6 fold (0.3 μM XN co-treated group) and 4.1 fold (0.5 μM XN co-treated group), respectively (one-way ANOVA, GraphPad Prism 5, *** p < 0.001, * p < 0.05, ns –statistically not significant). Scale bare = 100 μm

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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