Fig. 7

Fibronectin loss of function affects gliovascular interactions, gliosis, and microglial activity after amyloid toxicity in zebrafish brain. a Feature plots for fibronectin 1a (fn1a) and fibronectin 1b (fn1b) genes in zebrafish brain. b Violin plots in control and Aβ42-treated brains. fn1b is mainly expressed in vascular smooth muscle cells and immune cells and is upregulated with Aβ42. c, d Double IF for astroglia marker glutamine synthase (GS, red) and tight junction marker (ZO-1, green) in wild-type and fn1b^−/− animals. Individual fluorescent channels in c′, c′′, d′, and d′′. e, f Individual GS channels. g Quantification for colocalization of ZO-1 and GS. h Comparison of intensity measurements for GS. i, j Double IF for synaptic marker SV2 (green) and microglial marker l-Plastin (red) in wild-type and fn1b^−/− animals treated with Aβ42. Individual fluorescent channels in black–white channel. k Quantifications for synaptic density, total number of microglia, and activated microglia. Scale bars equal 25 µm