FIGURE

Figure 4

ID

ZDB-FIG-240315-78

Publication

Aksoy et al., 2024 - Zebrafish CCNF and FUS Mediate Stress-Specific Motor Responses

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Figure 4

Generating fus-deficient zebrafish lines. (A) Design of sgRNA targeting the RRM region of fus. (B) −10bp deletion at the TALEN target site resulting in a premature stop codon (*) (C) High resolution melting analysis (HRMA) curves showing WT (fus^+/+), heterozygous (fus^+/−) and homozygous (fus^−/−) zebrafish lines (D) RFLP assay showing the PCR amplicons of fus^+/+, fus^+/− and fus^−/− digested with DraI enzyme. (E) Quantification of fus^+/− and fus^−/− mRNA, relative to fus^+/+ mRNA in 4dpf embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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