FIGURE

Fig. 2

ID
ZDB-FIG-240306-61
Publication
Dennhag et al., 2024 - fhl2b mediates extraocular muscle protection in zebrafish models of muscular dystrophies and its ectopic expression ameliorates affected body muscles
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Fig. 2

fhl2b is upregulated in the EOM in response to desmin-related muscular dystrophy.

a Expression of myofiber-related DEGs for the following comparisons: 5 months desma−/−:desmb−/−vs WT EOMs (I), 5 months desma−/−:desmb−/−vs WT trunk (II), 20 months desma−/−:desmb−/−vs WT EOMs (III), 20 months desma−/−:desmb−/−vs WT trunk (IV), 5 months WT EOMs vs WT trunk (V), 5 months desma−/−:desmb−/− EOMs vs desma−/−:desmb−/− trunk (VI), 20 months WT EOMs vs WT trunk (VII) and 20 months desma−/−:desmb−/− EOMs vs desma−/−:desmb−/− trunk (VIII). bfhl2b expression in the abovementioned comparisons I-VIII. FHL2 antibody labeling of WT and desma−/−;desmb−/− cross-sectioned EOMs at c 5 and d 20 months. e FHL2 positive myofibers quantification of desma−/−;desmb−/− versus WT control EOMs in 5 (p = 0.017) and 20-monthold zebrafish (p = 3.7e−7), respectively and 5-months versus 20-months-old WT (p = 0.014) and desma−/−:desmb−/− (p = 0.005), respectively. Data is presented as median (line) and quartiles (dashed lines). f WT, plecb−/− and obscnb−/− 12-month-old EOM cross sections immunolabeled with FHL2 antibodies. g Human EOM cross section immunolabeled with DAPI/FHL2/laminin antibodies, dashed square indicates area enlarged below. h Western blot on human EOMs showing FHL2. i Mouse EOM cross section immunolabeled using DAPI/FHL2/laminin antibodies, dashed boxes indicate area enlarged below. j Western blot of mouse EOMs showing FHL2. Statistical analysis in b: Two-sided Wald test with B/H-correction, e: Two-sided t-tests with Welch correction. Scale bars in c, d, f, g, i: 50 µm, g, i bottom panel: 25 µm. White dashed lines outline the entire cross-section of the EOMs. Schematic images were adapted from https://www.biorender.com.

Expression Data
Gene:
Antibody:
Fish:
Anatomical Term:
Stage: Adult

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Adult

Phenotype Detail
Acknowledgments
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