FIGURE 2

Body length is affected in MZvgll4 larvae which could be related to epiboly progression delay. (A) Lateral views of wt and MZvgll4a larvae at 3dpf (left) and graph (B) showing the quantification of the body length from wt, MZvgll4a, MZvgll4b, MZvgll4l and MZvgll4a;MZvgll4b larvae at 3dpf. Note that MZvgll4a, MZvgll4b and MZvgll4a;MZvgll4b but not MZvgll4l mutants are shorter that the wt. Mann Whitney test, p < 0.0001. (C) The body length of MZvgll4a;MZvgll4b;MZvgll4l triple mutant larvae are also shorter than wt at 3dpf. Kruskal–Wallis test, p < 0.0001. (D) Schematic representation of the experimental design in (F). (E) The blebbistatin treatment at 4hpf during 30 min, delays epiboly progression in wt embryos (F) The treatment of wt embryos with blebbistatin at 4hpf during 30 min affects the body length of the larvae at 3dpf. Mann Whitney test, p < 0.0001. But not at 3hpf nor 5 hpf. Mann Whitney test, p = 0.2545 and p = 0.4396 respectively. T-test, p < 0.0001. Ns, not significant. ****p < 0.0001.