FIGURE

Figure 6

ID
ZDB-FIG-240206-44
Publication
Cudak et al., 2024 - Compartmentalization and synergy of osteoblasts drive bone formation in the regenerating fin
Other Figures
All Figure Page
Back to All Figure Page
Figure 6

Effects of mmp9+ and osterix+ cell ablation on fin regeneration

(A) 5 dpa fin regenerates of 3–5 dpa NFP-treated fin regenerates of mmp9:NTR, osterix:NTR, osterix:NTR x mmp9:NTR sibling zebrafish and osterix:CreERT2mCherry zebrafish, respectively. Fluorophore view images in high brightness and contrast settings (asterisks) to reveal dying cells in NTR+ zebrafish (same adjustments for osterix:CreERT2mCherry). White arrowhead, reduced regenerate length, black arrowheads, segment joints, black arrow, incomplete segment joint.

(B) Quantification of experiment shown in (A). One-way Anova (Tukey), excluding osterix:CreERT2 (non-sibling).

(C) IHC sections of NFP and BrdU treated 5 dpa osterix:mCherry and osterix:NTR x mmp9:NTR zebrafish fin regenerates. Arrowhead, BrdU+ cells in distal regenerate.

(D) Quantification of experiment shown in (C). Kruskal-Wallis. Epid, epidermis, Mes, mesenchyme, dist., 0–250 μm from regenerate tip, prox., 250 μm from regenerate tip to amp plane.

(E) Recovery treatment regimen.

(F) 5 and 7 dpa fin regenerates of 3–5 dpa NFP treated fin regenerates of osterix:NTR x mmp9:NTR zebrafish (DMSO treatment during recovery).

(G) Increase in regenerate length in mmp9:NTR, osterix:NTR, osterix:NTR x mmp9:NTR, and osterix:mCherry zebrafish during 2 days recovery (DMSO treatments). Dunnett’s T3, excluding osterix:mCherry (non-sibling).

(H) DMSO and SU5402 recovery treated 7 dpa fin regenerates of osterix:NTR x mmp9:NTR and osterix:mCherry zebrafish. Scale bars (A, F, H) 200 μm, (C) 20 μm. (B), (D), (G) Mean ± SD.
Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ iScience
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.