FIGURE

Fig. 6

ID

ZDB-FIG-240202-37

Publication

Nurcombe et al., 2023 - Plexina4 and cell survival in the developing zebrafish hindbrain

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Fig. 6

clu expressed in but not required for the formation of the floorplate. (A–D) In whole mount (A, C) and transverse sections (B, D) of 24 hpf (A, B) and 48 hpf (C, D) embryos, clu is expressed in the floorplate (arrows). At 48 hpf, clu mRNA is also present in the choroid plexus. (E, F) At 24 hpf (E) and 48 hpf (F) plxna4 (green) and clu (blue) mRNAs are expressed in cells adjacent to one another in the hindbrain, as evident in the overlay of comparable sections. (G–J) Uninjected control (G, I) and clu ATG morphant (H, J) embryos (N = 1, UIC n = 5, morphant n = 7) (G, H). Expression of foxa2, a marker of the floorplate, in whole mount at 24 hpf (N = 2, UIC n = 12, morphant n = 11) (I, J) and in transverse section at 48 hpf (K, L). A: anterior; cp: choroid plexus, D: dorsal, e: eye, fp: floorplate, hb: hindbrain, P: posterior, sc: spinal cord, V: ventral. Orientation in A for A, C, G–J and in B for B, D–F, K, L. Scale bar: 300 μm (A, C), 400 μm (G, H), 75 μm (B, D–F, K, L).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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