FIGURE

Fig. 7

ID

ZDB-FIG-240125-22

Publication

Gence et al., 2023 - Insulin signaling promotes neurogenesis in the brain of adult zebrafish

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Fig. 7

Intracerebroventricular injection leads to diffusion of the injected solution through the ventricular system. (a) Dorsal views before and after dissection of the skull allowing to see the Evans blue ICV injection. Arrows show the diffusion of Evans blue in the telencephalic and ventricle at the level of the optic tectum (TeO). (b) Ventral view showing the diffusion of the Evans blue within the hypothalamic ventricle (Arrow). (c–g) Fluorescence imaging performed in Tg(GFAP::GFP) zebrafish showing Evans blue (red) staining in the ventricle and periventricular layer of the dorsomedian telencephalon (Dm), ventral telencephalon (Vv), periventricular pretectal nucleus (PPv), anterior hypothalamus (Hv), TeO, valvula of the cerebellum (Vce) and caudal hypothalamus around the lateral and posterior recess (LR PR). (h) HDLs labeled with DilC18 (red) demonstrating that the injection at the junction of the telencephalon/rhombencephalon well results in the diffusion of HDLs in the anterior part. Note that cell nuclei are counterstained with DAPI.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Comp. Neurol.