Figure 3

Optn and p62 can protect against Mm independently of the autophagy modulator Dram1. (A) Overexpression of optn in dram1 wildtype and mutant background. In the experimental workflow, optn mRNA was injected into dram1 +/+ and -/- embryos at the one cell stage, followed by injection of 200 CFU of Mm into the blood island at 28 hpf and assessment of bacterial burden at 4 dpi (representative images shown). Quantification shows that optn overexpression could decrease bacterial burden independent of dram1. (B) Overexpression of p62 in dram1 wildtype and mutant background. Overexpression of p62 was studied by an experimental workflow analogous to that in (A) and found to decrease bacterial burden independent of dram1. Data are displayed as percentage difference to the control group set at 100% and are accumulated from three independent infection experiments (each data point representing an individual embryo), indicated with different colors. ns, non-significant, * p<0.05, **p<0.01, ***p<0.001.