Canonical ligands of Csf1r, including Csf1 and Il34, are neither required nor sufficient for HSPC expansion. (A) WISH of cmyb at 3 dpf in siblings and csf1a, csf1b and il34 single, double and triple mutants. Scale bars: 50 μm. (B) Quantification of cmyb+ HSPCs in CHT at 3 dpf in siblings and csf1a, csf1b and il34 single, double and triple mutants. n=29 (siblings); 23 (csf1amut); 22 (csf1bmut); 4 (il34mut); 36 (csf1amutcsf1bmut); 8 (csf1amutil34mut); 8 (csf1bmutil34mut); 11 (csf1amutcsf1bmut il34mut). (C) WISH of cmyb at 4 dpf in ABSR (wild type) (n=9) and Tg(hsp70:csf1a) fish (n=52). Both embryos were heat shocked at 37°C in a water bath for 1 h at 2 dpf, 2.5 dpf and 3 dpf. Red arrowheads indicate cmyb+ HSPCs. (D) WISH of cmyb at 4 dpf in ABSR (n=8) and Tg(hsp70:csf1b) fish (n=50). Both embryos were heat shocked at 37°C in a water bath for 1 h at 2 dpf, 2.5 dpf and 3 dpf. Red arrowheads indicate cmyb+ HSPCs. (E) WISH of cmyb at 3.5 dpf in ABSR (n=15) and Tg(hsp70:il34) fish (n=27). Both embryos were heat shocked at 37°C in a water bath for 1 h at 2 dpf, 2.5 dpf and 3 dpf. Red arrowheads indicate cmyb+ HSPCs. Data are presented as mean±s.e.m. ns, not significant.
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