Fig. 5

P. aeruginosa infections in zebrafish embryos and in the A549 cell line (A) Kaplan-Meier zebrafish embryo survival curves on infection with P. aeruginosa. One-day-old embryos were injected with ∼700–1000 CFU of the P. aeruginosa PAO1 wild-type strain or with the indicated isogenic mutant. Uninfected control (non-injected) is shown. Data are means ± SD of four biologically independent replicates (N = 4) with ∼20 embryos/group in each replicate. The hazard ratio (HR), the 95% confidence interval ratio (95% CI), and the P-values (Log Rank Mantel-Cox) of each curve with respect to the reference PAO1 curve (HR = 1) are indicated. (B) A549 cell viability. The P. aeruginosa PAO1 wild-type strain and the indicated isogenic mutant were co-incubated with the eukaryotic cells. Formazan production on addition of the MTT tetrazolium salt was determined spectrophotometrically at 620 nm. Uninfected cells (white bar) were used as control. Data are means ± SD from five biological replicates (N = 5). P-values were calculated by two-tailed t-test and are represented in the graphs by ns, not significant; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001; and ∗∗∗∗, p < 0.0001. Brackets indicate the comparison to which the P-value applies.