FIGURE

Fig. 3

ID

ZDB-FIG-230920-11

Publication

Verwilligen et al., 2023 - Zebrafish as outgroup model to study evolution of scavenger receptor class B type I functions

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Fig. 3

Fig. 3. Deficiency of SCARB1 does not alter fluorescently-labeled lipoprotein uptake in the caudal vein, total plasma cholesterol, and stress-induced cortisol levels in zebrafish (A) Uptake of DiO-HDL (24 h post injection)(green), Bodipy-LDL and DiO-oxLDL (30-60 min post injection)(green) by macrophages (purple) in WT and scarb1^−/− zebrafish larvae. Magnification 40× (B) Free serum cholesterol (FC) (C) total serum cholesterol levels (TC) and (D) FC/TC ratio of WT sibling (n_WT = 11 pooled samples of 2) and homozygous scarb1^−/− zebrafish (n_-1nt = 5 and n_+2nt = 6 pooled samples of 2) measured with enzymatic colorimetric assay. (E) Cortisol levels of WT (n_WT = 6 pooled samples of 30) and scarb1^−/− zebrafish larvae (n_-1nt = 6 pooled samples of 30, n_+2nt = 6 pooled samples of 30) at baseline and after net stress. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Expression Data

Gene:	mCherry
Fish:	ump2Tg (AB/TL) scarb1^ibl4/ibl4; ump2Tg (AB/TL) scarb1^ibl5/ibl5; ump2Tg (AB/TL)
Conditions:	chemical treatment by injection: low-density lipoprotein chemical treatment by injection: high-density lipoprotein chemical treatment by injection: oxidised LDL
Anatomical Term:	macrophage
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TL scarb1^ibl4/ibl4 (AB/TL) scarb1^ibl5/ibl5 (AB/TL) scarb1^ibl4/ibl4; ump2Tg (AB/TL) scarb1^ibl5/ibl5; ump2Tg (AB/TL)
Conditions:	chemical treatment by injection: low-density lipoprotein chemical treatment by injection: high-density lipoprotein chemical treatment by injection: oxidised LDL stress by handling
Observed In:	blood plasma cholesterol macrophage high-density lipoprotein particle clearance macrophage low-density lipoprotein particle clearance macrophage oxidised low-density lipoprotein particle clearance whole organism cortisol
Stage Range:	Day 4 to Adult

Phenotype Detail

Acknowledgments

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Reprinted from Biochimica et biophysica acta. Molecular and cell biology of lipids, 1868(6), Verwilligen, R.A.F., Mulder, L., Araújo, P.M., Carneiro, M., Bussmann, J., Hoekstra, M., Van Eck, M., Zebrafish as outgroup model to study evolution of scavenger receptor class B type I functions, 159308, Copyright (2023) with permission from Elsevier. Full text @ BBA Molecular and Cell Biology of Lipids