Fig. 7

(A) Representative ventral images show cartilage structures in the anterior region of -1.4col1a1:egfp zebrafish larvae at 3 dpf for control, morpholino (MO), and MO rescued with WT human MAP4K4 mRNA. Adjoining white dashed lines indicate the ceratohyal (CH) angle; vertical dashed line indicates the distance from Meckel’s cartilage (MK) to CH. CB, ceratobranchial arches. (B and C) Quantification of CH angle and CH to MK distance measurements on ventral green fluorescent protein–positive (GFP⁺) images acquired at 3 dpf [as shown in (A)]; n = 35 to 40 larvae per batch, repeated. (D) Representative ventral images of cardiac chambers of cmlc2:egfp zebrafish larvae at 2 dpf. (E and F) Quantification of atrium and ventricle area measured on ventral images at maximum diastole [as shown in (D)]; n = 38 to 45 larvae per batch, repeated. For (B), (C), (E), and (F), each condition is normalized to its respective experimental control and expressed as a percentage. Scale bars, 300 (A) and 200 μm (D). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; n.s., not significant, by pairwise Student’s t test compared to vehicle. Error bars represent the SEM.