FIGURE

Figure 1

ID

ZDB-FIG-230822-1

Publication

Altbürger et al., 2023 - CRISPR/Cas9-based QF2 knock-in at the tyrosine hydroxylase (th) locus reveals novel th-expressing neuron populations in the zebrafish mid- and hindbrain

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Figure 1

Knock-in of QF2 into the th locus. (A) Schematic of the knock-in strategy used for the generation of th^Tg(2A−QF2), which is based on a previously published strategy (Li et al., 2015). Successful knock-in leads to an in-frame fusion of Th with E2A-QF2, which is cleaved after translation, releasing QF2. QF2 translocates to the nucleus and activates a gene of interest (GOI) downstream of a QUAS regulatory element. (B) Maximum intensity projection of a 2-photon confocal image stack of an in vivo recorded th^{m1512Tg(2A−QF2)}; Tg(QUASr:GFP)c403 larva at 5 dpf in a dorsal view. (C) Maximum intensity projection of the embryo shown in (B) in a sagittal resliced view. Anterior is to the left. Scale bar: 100 μm. Due to very strong intensity differences in cytoplasmic GFP fluorescence of somata vs. axons, and in order to visualize both somata and projections, we needed to record under conditions that oversaturate pixels in many somata. AR, area postrema; DAC, dopaminergic cluster; LC, locus coeruleus; Mb, midbrain; MHB, midbrain–hindbrain boundary; MO, medulla oblongata; OB, olfactory bulb; ORR, optic recess region; Pr, pretectum; rHb, rostral hindbrain; Sp, subpallium; sym, sympathetic neurons.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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