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aldh1a2 upregulation in lamb1a mutants is contractility dependent. (A-D) mRNA in situ hybridisation analysis of aldh1a2 expression in sibling and lamb1aΔ25 mutant embryos at 30 hpf (A,B) and 55 hpf (C,D). lamb1aΔ25 mutants exhibit an upregulation of aldh1a2 expression in the endocardium at both stages (arrowheads in B,D; 30 hpf: n=14/20; 55 hpf: n=16/17) compared with siblings (30 hpf: n=65/66; 55 hpf: n=48/53). (E-J) mRNA in situ hybridisation analysis of aldh1a2 expression at 55 hpf in sibling and lamb1aΔ25 mutant embryos, either uninjected (E,H), injected with tp53 MO (F,I) or co-injected with tp53 MO and tnnt2a MO (G,J). The upregulation of aldh1a2 expression in the endocardium of lamb1aΔ25 mutants (H: n=12/14; I: n=12/16) is lost upon injection with tnnt2a MO (J: n=12/18). Black-dashed outlines indicate the heart. Scale bars: 50 μm. A, atrium; V, ventricle.
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