Fig. 4

A single copy of the miR-430 promoter including stem cell transcription-factor-binding sites is sufficient to activate reporter expression during the minor wave of genome activation

(A) Browser views showing CAGE-seq signal (top track, blue bar) marking the position of the TSS (red arrowheads). ChIP-seq signal of Nanog (purple), Pou5f1 (green), and Sox2 (pink) are shown below. Bottom annotation tracks show the miR-430 gene features, with the core promoter (red), the precursor triplet (blue), and the position of predicted pluripotency-factor-binding sites. Purple dash line marks the Nanog binding site in the core promoter region close to the peak of the Nanog ChIP-seq signal.

(B) Top: schematic of the generation of miR-430 promoter-containing stable transgenics reporter lines using the PhiC31 site specific integration system. Bottom: expression of the reporter miR-430 promoter (left) and γ-crystalline promoter (right), detected by RT-PCR at the indicated stages. Abbreviations: TSS, transcription start site; γ-Cry, γ-crystalline C.

See also Figure S3.