Fig. 3
- ID
- ZDB-FIG-230710-45
- Publication
- Hadzhiev et al., 2023 - The miR-430 locus with extreme promoter density forms a transcription body during the minor wave of zygotic genome activation
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Promoter architecture features of minor and main wave ZGA genes (A) Schematic comparing sharp and broad promoter architectures. (B) Sequence logo of 80 miR-430 genes showing the −35 to +15 region relative to the dominant TSS. (C) Genome browser views showing promoter shape as defined by CAGE-seq signal for miR-430, and example genes for the minor (mxtx2) and the major (rgma) wave gene sets. (D) Promoter architecture comparison between the 4 gene sets. Left: sequence logos of the −35 to +15 region relative to the TSS, right: percentage of the genes with sharp (red) and broad (blue) promoter in each gene set, determined by the IQW of the corresponding CAGE consensus cluster as demonstrated (A). (E) Chromosomal plot showing the location of sharp (red) and broad (blue) promoter znfs on chr4 and the location of the miR-430 cluster (orange). (F and G) Expression activity of sharp and broad promoter znfs determined by CAGE-seq (F) and EU nascent RNA-seq (G). (H and I) Comparison of the promoter motifs/architecture of the sharp (H) and broad (I) znfs. (J and K) Distribution of H3K4me3 signal around the promoter for the sharp (K) and broad (J) znfs. Abbreviations: TSS, transcription start site; IQW, interquantile width. |
Reprinted from Developmental Cell, 58, Hadzhiev, Y., Wheatley, L., Cooper, L., Ansaloni, F., Whalley, C., Chen, Z., Finaurini, S., Gustincich, S., Sanges, R., Burgess, S., Beggs, A., Müller, F., The miR-430 locus with extreme promoter density forms a transcription body during the minor wave of zygotic genome activation, 155170.e8155-170.e8, Copyright (2023) with permission from Elsevier. Full text @ Dev. Cell