Fig. 1

The differentiation of intestine, liver, and exocrine-pancreas is impaired in wdr5^−/− mutant embryos

a Whole-mount in situ hybridization (WISH) of wild type (WT) and wdr5^−/− mutant embryos at 3 dpf to show the expression of differentiation genes for different digestive organs including: the fabp10a and gc for liver, prss1and ela2l for exocrine pancreas, fabp2 and chia.1 for intestine as indicated. b Hematoxylin-Eosin (H&E) staining of cryosections to show liver (L), intestine (I) and exocrine pancreas (P) in WT and wdr5^−/− mutant embryos at 3 dpf. Scale bar: 40 μm. c WISH of wdr5 in WT at one cell stage, 1, 2 and 3 dpf. d Heatmap of genes related to endodermal organ differentiation in WT and wdr5^−/− mutant embryos at 3 dpf. e Volcano plot to show differential expressed regulators of endodermal organ development between WT and wdr5^−/− mutant embryos at 3 dpf (wdr5^−/− mutant vs WT, |log₂FoldChange | ≥ 1, P_adj < 0.05). f WISH of WT and wdr5^−/− mutant embryos at different time points with different digestive organ development regulators including: pan-endodermal marker foxa3, hepatic marker prox1a and intestine marker cdx1b as indicated. n indicates the number of zebrafish embryos in each group.