FIGURE

Figure 3

ID

ZDB-FIG-230629-59

Publication

Mazumdar et al., 2023 - Loss of Nicotinamide nucleotide transhydrogenase sensitizes embryos to ethanol-induced neural crest and neural apoptosis via generation of reactive oxygen species

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Figure 3

nnt mutants appear sensitized to ethanol teratogenesis from 6 to 24 hpf. Embryos were treated with 1% ethanol at different timepoints (A–H) to establish a critical period of ethanol teratogenesis. All images are ventral views with anterior to the left. Control (unexposed) wildtype and mutant (A, E) appear phenotypically normal. Wildtype embryos dosed across all timepoints (B–D) appear phenotypically normal. (F) Mutant embryos dosed at 6–24 hpf had stark craniofacial defects consisting of a hypoplastic Meckel’s cartilage (yellow arrow), deformed ceratohyal (red arrow), microphthalmia, and microcephaly. (G) Mutant embryos dosed at 24–48 hpf had no apparent defects in craniofacial morphology, but did have microcephaly and reductions in the size of skeletal elements. (H) Mutant embryos dosed at 48–72 hpf had no apparent defects. (I) Quantification of the percentage of fish with craniofacial defects in each group. (Fischer’s exact test, n ≥ 20 per group, **p < 0.01, ****p < .0001). The most highly statistical difference was observed at 6–24 hpf. Mut: mutant, Het: heterozygote, Wt: wildtype.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nnt^au111/au111 (AB)
Condition:	chemical treatment by environment: ethanol
Observed In:	ceratohyal cartilage eye head Meckel's cartilage
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neurosci.