FIGURE

Figure 2

ID

ZDB-FIG-230629-58

Publication

Mazumdar et al., 2023 - Loss of Nicotinamide nucleotide transhydrogenase sensitizes embryos to ethanol-induced neural crest and neural apoptosis via generation of reactive oxygen species

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Figure 2

Loss of nnt sensitizes embryos to ethanol teratogenesis. (A–D) Alcian Blue/Alizarian Red-stained zebrafish at 5 dpf treated from 6 hpf- 5 dpf. All images are ventral views with anterior to the left. (A,B) Control and ethanol-treated wildtypes and (C,D)nnt mutants. Both the untreated and 1% ethanol dosed wt embryos appear phenotypically normal. (C) Untreated nnt mutant with a typical phenotype akin to wt. (D) Ethanol-exposed nnt mutant zebrafish with aberrant craniofacial phenotype consisting of a hypoplastic Meckel’s cartilage (yellow arrow), deformed ceratohyal (red arrow), microphthalmia, and microcephaly. (E) Quantification of the percentage of fish with craniofacial defects in each group (Fischer’s exact test, n ≥ 20 per group, ****p < .0001). Only ethanol-treated mutants displayed craniofacial defects, a statistically significant increase. EtOH: ethanol, Ctrl: control, mut: mutant, het: heterozygote, Wt: wildtype.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nnt^au111/au111 (AB)
Condition:	chemical treatment by environment: ethanol
Observed In:	ceratohyal cartilage eye head Meckel's cartilage pharyngeal arch cartilage
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neurosci.