Figure 7

Combined treatment with BIBR and fludarabine (FLU) or cyclophosphamide (CY) inhibited the growth of EBV-immortalized and fully transformed B cells xenografted in zebrafish. 4134/Late (A) and BL41 (B) cells were pre-treated with 30 μM BIBR (pre-BIBR) or DMSO (pre-DMSO) as a control for 16 h, stained with CM-DiI, and xenotransplanted in the yolk sac of 72 hpf embryos. Twenty-four hours post-xenotransplantation, xenografted embryos were divided into groups untreated (NT) or treated with FLU or CY. The percentage of tumour cells in engrafted embryos was determined at 24, 48, and 72 h post-treatment (hpt) by flow cytometric analysis. Data represent the mean and SD (bar) from three separate experiments with 10 embryos per group. ns: not significant, *, ^▼ and • p < 0.05; ** and ^▼▼ p < 0.01; *** p < 0.001, where (*) asterisks represent the statistical significance vs. the pre-DMSO NT group; the (▼) symbol shows the statistical significance of the combined treatment with BIBR and CY or FLU vs. the respective drug treatment alone and the (•) symbol shows the statistical significance of the combined treatment with BIBR and CY or FLU vs. the BIBR treatment alone.