FIGURE
Figure 6
- ID
- ZDB-FIG-230401-8
- Publication
- Pezzotta et al., 2023 - Combined Inhibition of Hedgehog and HDAC6: In Vitro and In Vivo Studies Reveal a New Role for Lysosomal Stress in Reducing Glioblastoma Cell Viability
- Other Figures
- All Figure Page
- Back to All Figure Page
Figure 6
Impairment of the autophagic process in the zebrafish model with Hh hyper activation and in the zicras mutants. (A,B) Confocal images of the trunk-tail region of Tg(CMV:eGFPmap1Lc3b) embryos injected with (A) rfp-mRNA or (B) shh mRNA. (C) Count of the Lc3b puncta of rfp- and shh-mRNA injected embryos. Scale bar indicates 100 μm. (D–G) Expression analyses of (D) gli1 and genes involved in autophagy activation, including (E) dram1; (F) atg4a, and (G) atg4c, derived from the analyses of wild-type and zicras (tumor) zebrafish brains ( GSE74754). Data are presented as mean ± standard deviation. cpm—counts per million. Unpaired t-test with Welch correction. *** p < 0.001; ** p < 0.01; * p < 0.05. Scale bar indicates 100 μm. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Int. J. Mol. Sci.